Over-flow cell-washing method to decrease false negative phenomenon in antiglobulin tests by automatic cell washers.

False-positive antiglobulin tests in healthy subjects and in hospital patients.

An antiglobulin reagent containing anti-IgG, -C4c, -C4d, -C3c, and -C3d was used to test red blood cells from healthy subjects and from patients in hospital. In tests read macroscopically on opal tiles, no positive reactions were found in the healthy subjects. Positive reactions were, however, observed in 7% of the hospital patients. Further tests with monospecific reagents showed the positive ...

Washing Insulators by Ultrasound Sonication Method to Remove Hard Sediments Caused by Dust and Aerosols

Correct and timely maintenance of power lines and substations has an important impact on the operation and optimization of the production cycle and distribution of electricity. In this way, proper and timely maintenance and repair of these lines can reduce losses, sudden defects, and thus reduce and optimize consumption. One of the most important components of transmission and distribution netw...

Cell Envelope Structure and Functions in Gram-negative Bacteria

Avoiding False Positive Antigen Detection by Flow Cytometry on Blood Cell Derived Microparticles: The Importance of an Appropriate Negative Control

BACKGROUND Microparticles (MPs), also called microvesicles (MVs) are plasma membrane-derived fragments with sizes ranging from 0.1 to 1μm. Characterization of these MPs is often performed by flow cytometry but there is no consensus on the appropriate negative control to use that can lead to false positive results. MATERIALS AND METHODS We analyzed MPs from platelets, B-cells, T-cells, NK-cell...

Estimating false positive and false negative error rates in cervical cell classification.

The performance of a cell recognition system on unknown data is often estimated in terms of its error rates on a test set. This paper investigates methods for producing estimates of error rates in cervical cell classification. Classification performance curves calculated using these methods are given for several classification schemes used to classify 1500 cervical cells.